Table 1. Annotation clusters of differentially regulated genes.
| Threefold embryo | Score | L1 | Score | Adult | Score |
|---|---|---|---|---|---|
| Cilium assembly/dauer | 10.67 | Cuticle/collagen | 12.04 | Signal peptide | 3.53 |
| Cilium biogenesis/protein transport | 4.85 | Cilium assembly/behavior | 5.21 | Aging | 2.99 |
| Mitosis/cell cycle | 4.04 | Cilium assembly/dauer | 4.17 | Proteolysis | 1.69 |
| Vulval location/dauer entry | 3.85 | Lipid modification | 3.31 | Lipase | 1.52 |
| Glutathione transferase | 3.66 | Disulfide bond/cuticle | 2.08 | 172 IDs, 17 clusters | |
| Protein processing/ubiquitin | 3.24 | Signal peptide | 1.85 | ||
| Cilium morphogenesis/behavior | 3.21 | Aging | 1.82 | ||
| Glucuronidation | 3.08 | Proteolysis | 1.52 | ||
| Cytoskeleton | 2.86 | Neuropeptide | 1.48 | ||
| Endoplasmic reticulum | 2.54 | 364 IDs, 37 clusters | |||
| Spindle/polar body extrusion | 2.48 | ||||
| Nucleosome | 2.45 | ||||
| ER lumen | 2.22 | ||||
| Histone | 2.03 | ||||
| Oxidoreductase | 2.03 | ||||
| Kinetochore/centromere | 1.97 | ||||
| Tetratricopeptide-like helical | 1.91 | ||||
| Cyclin | 1.90 | ||||
| Glucose/ribitol dehydrogenase | 1.77 | ||||
| EF-hand domain | 1.68 | ||||
| ATP-binding | 1.54 | ||||
| 909 IDs, 21 clusters > 1.5 |
Gene annotation clusters of differentially regulated genes. Genes identified as putative targets of daf-19 in threefold stage embryos (Phirke et al. 2011), L1 larvae, and 2-day-old adults were clustered using the DAVID Bioinformatics Resource 6.7 with a medium classification stringency (Huang et al. 2009). Clusters with scores of ≥ 1.5 are shown. IDs, identifiers; DAVID, database for annotation, visualization and integrated discovery; ER, endoplasmic reticulum.