Table 3. daf-19 isoform-specific target gene regulation.
| Expression in indicated worm genotype | |||||||
|---|---|---|---|---|---|---|---|
| Gene name | Gene ID | Wild-type daf-19 | daf-19 (tm5562) | daf-19(0) + daf-19c | daf-19(0) + daf-19a | Interpretation | |
| Target gene expression activated in the presence of daf-19 | |||||||
| asic-2 | T28F4.2 | Activated | Activated | Rescues activation | Ectopic repression | DAF-19C activates target gene; DAF-19A overexpression represses | |
| spg-20 | F57B10.9 | Activated | Activated | Rescues activation | Ectopic repression | DAF-19C activates target gene; DAF-19A overexpression represses | |
| ddn-1 | B0507.10 | Activated | Activated | Rescues activation | No rescue | DAF-19C activates target gene | |
| eppl-1 | T01B11.2 | Activated | Activated | Rescues activation | N/A | DAF-19C activates target gene | |
| del-4 | T28B8.5 | Activated | Activated | Rescues activation | Partial rescue | DAF-19C activates target gene | |
| mapk-15 | C05D10.2 | Activated | Activated | Rescues activation | Ectopic repression in head | DAF-19C activates target gene; DAF-19A overexpression represses | |
| Target gene expression repressed in the presence of daf-19 | |||||||
| skr-12 | C52D10.6 | Repressed | Activated | Activated | Ectopic repression | Repression requires DAF-19A; DAF-19A overexpression represses expression | |
| gakh-1 | F46G11.3 | Repressed | Activated | Activated | Rescues repression | Repression requires DAF-19A | |
| rgs-8.1 | F52D2.2 | Repressed | Repressed | Rescues repression | Rescues repression | Either isoform can repress | |
| mapk-15 | C05D10.2 | Repressed | Activated | Activated | Repressed in head | Repression requires DAF-19A | |
Isoform-specific control of target gene expression. The average expression phenotype of daf-19 target genes is shown in a daf-19(tm5562) mutant background (lacking functional DAF-19A) and in daf-19 null (m86) worms expressing either daf-19c or daf-19a from their endogenous promoters. ID, identifier; N/A, unc-122p::gfp co-injection marker not detectable due to target gene expression in muscle.