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. 2018 Jan 5;208(3):963–976. doi: 10.1534/genetics.117.300529

Figure 1.

Figure 1

Isw1 prevents CAG-85 repeat expansions. (A) A CAG-85 tract is on the right arm of the URA3-YAC (YAC CF1), where the URA3 gene is 198 bp from CAG-85 and 4.2 kb from the telomere. Repeat length was measured by PCR amplification using primers (black arrows) upstream and downstream from the repeat, with an intact CAG-85 repeat resulting in a 414-bp product. The frequency of CAG-85 expansions was measured in strains lacking (B) ISW1 or other genes encoding chromatin remodeling proteins (ISW2 and CHD1), (C) genes encoding subunits of Isw1 complexes Isw1a (IOC3) or Isw1b (IOC2 and IOC4) individually or in combination with deletion of another subunit, and (D) genes encoding proteins with known functions of recruiting Isw1 to chromatin (SET1, SET2, and CBF1). Expansion frequencies were tested for significant deviation from wild-type (WT) frequency using Fisher’s exact test, * P < 0.05 and ** P < 0.01.