Skip to main content
. 2018 Jan 16;208(3):991–1007. doi: 10.1534/genetics.118.300686

Figure 4.

Figure 4

RAD-51 disassembly is compromised in germ lines lacking Mi2. (A) Schematic of the C. elegans germ line. Nuclei divide mitotically in the proliferative zone (PZ), undergo double-strand break (DSB) formation and homolog pairing in the transition zone (TZ), and repair DSBs in early to midpachytene (EP–MP). In wild-type worms, DSB repair is complete by late pachytene (LP), and chromosomes condense in diplotene (DP) through diakinesis (DI). Gray coloring denotes regions assayed for RAD-51 foci. Total rows of nuclei in these regions were counted and divided equally into four stages (indicated by dashed lines), starting in mid-TZ, as determined by DAPI morphology. (B) Quantification of total RAD-51 foci in TZ–LP nuclei in wild-type (N2) (red), chd-3(eh4) (yellow), let-418(n3526) (dark blue), and let-418(n3536);chd-3(RNAi) (light blue). A minimum of three germ lines were scored for each genotype. Statistical comparisons between each mutant and wild-type at corresponding stages were performed using a two-tailed Mann–Whitney test. * P ≤ 0.005 and ** P ≤ 0.0001. (C–F) Representative images of RAD-51 foci in TZ (C) through LP (F) in N2, let-418(n3536), chd-3(eh4), and chd-3(RNAi);let-418(n3536). Germlines were stained with RAD-51 (yellow) and counterstained with DAPI (blue). Bar, 5 μM. Each experiment assessing RAD-51 in chd-3(RNAi);let-418(n3536) germlines was performed alongside N2 and let-418(n3536) plus L4440 empty vector controls (data not shown).