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. 2018 Jan 16;208(3):991–1007. doi: 10.1534/genetics.118.300686

Figure 6.

Figure 6

Absence of nonhomologous end joining (NHEJ) exacerbates repair defects in let-418 mutants. (A) Quantification of RAD-51 foci per late pachytene nucleus in cku-80(ok861), let-418(n3536), cku-80(ok861);let-418(n3536);chd-3(eh4), and cku-80(ok861);chd-3(eh4) mutants. Statistical comparisons between single and double mutants were performed using a two-tailed Mann–Whitney test. * P ≤ 0.02 and ** P ≤ 0.0001. (B) Representative images of DAPI staining bodies in diakinetic (DI) nuclei in each mutant, which were quantified in (C): cku-80(ok861), (n = 30), cku-80(ok861);let-418(n3536), (n = 19), and chd-3(RNAi);cku-80(ok861);let-418(n3536), (n = 26). Number of visibly stained bodies is indicated in upper left corner of image. Arrowheads indicate extra DAPI-stained bodies, and circle indicates region with multiple fragmented chromosomes. Bar, 2 μM. Fragmentation (more than six DAPI bodies) was assessed using a Fisher’s exact test and was significant for the following comparisons: let-418 vs. cku-80;let-418: P = 0.0002; let-418 vs. chd-3(RNAi)cku-80;let-418: P = 0.0010; and cku-80 vs. chd-3(RNAi);cku-80;let-418: P = 0.0425. Chromosome fusions (less than six DAPI bodies) were identical between all three mutants: cku-80 vs. cku-80;let-418: P = 0.3478 and cku-80 vs. chd-3(RNAi);cku-80;let-418: P = 0.4304.