Fig. 6.

Real-time evaluation of ischemia-reperfusion model using PAOI. (a) B-mode $\mathrm{CR}-{\mathrm{sO}}_2$ map at baseline condition (no PBS injection-no IOP increase); a 25-gauge needle is inside the anterior segment of eye. Red and yellow dotted rectangles show the retina and control areas, respectively. The $\mathrm{CR}-{\mathrm{sO}}_2$ is $68.76\pm 2.34$ at baseline (no IOP increase; $10.5\pm 0.33\mathrm{mm}\mathrm{Hg}$). (b) The $\mathrm{CR}-{\mathrm{sO}}_2$ map 5 s after injection of PBS in the eye. Here, the change in the retina map to blue indicates a decrease in $\mathrm{CR}-{\mathrm{sO}}_2$ after 5 s of IOP. (c) $\mathrm{CR}-{\mathrm{sO}}_2$ gradients after injection of PBS (15 s after start of injection). The IOP increased to $46.5\pm 2.54\mathrm{mm}\mathrm{Hg}$, and the $\mathrm{CR}-{\mathrm{sO}}_2$ decreased to $12.3\pm 3.54$. (d) $\mathrm{CR}-{\mathrm{sO}}_2$ map in reperfusion condition. The imaging plane is different from other three because we had to remove the transducer and measure the IOP with a Tonopen. $\mathrm{CR}-{\mathrm{sO}}_2$ recovered to $58.87\pm 3.23$ after the IOP returned to $16.55\pm 1.67\mathrm{mm}\mathrm{Hg}$. (e) Real-time monitoring of $\mathrm{CR}-{\mathrm{sO}}_2$ and ${\mathrm{sO}}_2$ using PAOI and pulse oximetry. The $\mathrm{\Delta }$ shows ${\mathrm{sO}}_2$ difference between baseline and ischemia. (f) Statistical analysis of $\mathrm{CR}-{\mathrm{sO}}_2$ for five eyes under different conditions including baseline, ischemia, and reperfusion using PAOI. The PAOI shows sixfold decrease in $\mathrm{CR}-{\mathrm{sO}}_2$ during ischemia ($n=5$). There was a very significant change between baseline and ischemia and a significant different in $\mathrm{CR}-{\mathrm{sO}}_2$ from baseline to reperfusion. (g) Statistical analysis of IOP for five eyes at baseline, ischemia, and reperfusion using Tonopen. There is negative correlation between IOP and $\mathrm{CR}-{\mathrm{sO}}_2$. Error bars shows the standard deviation of five different eyes. Here, * indicates $p<0.05$ and ** indicates $p<0.0001$.