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. Author manuscript; available in PMC: 2019 Feb 21.
Published in final edited form as: Curr Protoc Immunol. 2018 Feb 21;120:24.2.1–24.2.12. doi: 10.1002/cpim.46

Figure 1. Skull thinning platform used for two-photon imaging.

Figure 1

A custom metal imaging platform consisting of a base, two support posts, a brace, and a rubber O-ring to contain aCSF can be quickly constructed for two-photon imaging studies of mice with thinned skull windows. The support posts should be threaded and have nuts to fasten the metal brace that is glued directly to the skull bone. The thinned skull window should be created and imaged within the hole cut in the center of the metal brace.