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. Author manuscript; available in PMC: 2018 Nov 29.
Published in final edited form as: Converg Sci Phys Oncol. 2017 Nov 29;3:044001. doi: 10.1088/2057-1739/aa9263

Figure 7.

Figure 7

Matrix stiffness and VEGF stimulate actin stress fiber formation in endothelial cells. (A) Phalloidin staining and corresponding fluorescence intensity heat maps of sub-confluent HUVECs on 1 or 10 kPa collagen-coated substrates under control conditions (upper panel) or 15 min VEGF stimulation (lower panel). Cells were counterstained with DAPI. Heat maps represent low (0) to high (1) intensity (A.U.). Insert is a 3x zoom of the boxed region. Images were acquired with identical exposure settings (Scale bars, 20 μm). (B) Quantification of the average number of stress fibers per cell for each condition. Plot is mean ± SE. N = 3 independent experiments, at least 30 cells per condition. * p<0.05, *** p<0.001 from ANOVA with post hoc Tukey’s test.