Fig 1. Hepatic histology scores.

Hepatic histology scores graded semi-quantitatively according to Kleiner et. al. non-alcoholic fatty liver disease (NAFLD) activity score (NAS), steatosis (0–3), lobular inflammation (0–2), ballooning (0–2) and fibrosis (0–4) in isocaloric controls, high-fat, high-cholesterol (HFHC) controls, two-week NPC2 (treatment) and four-week NPC2 (prevention) animals. No animal had significant histological fibrosis on HE (Panel A) or Masson trichrome staining (Panel B). *: P < 0.05 compared with Isocaloric Controls. #: P < 0.05 compared with HFHC Controls.