Fig 7. Canonical binding site potency.

Cumulative distributions of average interaction scores for all 7990 miRNA-3’ UTR combinations probed. (A) according to the presence of canonical binding site patterns. Combinations with multiple canonical binding site patterns have lower interaction scores than combinations with a single pattern, that in their turn have lower scores than combinations without canonical binding site patterns. All distributions are significantly different from one another (one-sided Kolmogorov-Smirnov p-values < 0.001 after Benjamini-Hochberg multiple testing correction). (B) according to the presence of different types of canonical binding site patterns. Combinations with at least one 8mer pattern, have lower interaction scores than combinations with at least one 7mer-m8, one 7mer-A1 and one 6mer pattern, respectively (combinations with multiple types of binding site patterns are considered in all respective distributions). Notably, the presence of multiple 8mer patterns produces the largest shift in distribution. All distributions are significantly different from one another (one-sided Kolmogorov-Smirnov p-values < 0.01 after Benjamini-Hochberg multiple testing correction). (C) according to the presence of 3’ supplementary pairing. Combinations harboring canonical binding site patterns with 3’ supplementary pairing have lower interaction scores than those without. All distributions are significantly different from one another (one-sided Kolmogorov-Smirnov p-values < 0.001 after Benjamini-Hochberg multiple testing correction).