Table 1. Distinct metastatic lesions originating from a common cell line exhibit niche-dependent molecular signatures.
PROTEINS UPREGULATED IN ADRENAL-TROPIC MCF7-6124A CELLS | |||
---|---|---|---|
Protein | Fold Change | Pathway activated | Clinical relevance |
N-Cadherin | 4.75 | Sustains activation of ERK and activates MMP-9 production | Promotes invasive activity and increases tumor metastasis |
ADAM17 | 6.5 | Activates EGFR-MEK-ERK pathway and upregulates MMP-2 and MMP-9, stimulates the release of TGFα | Increases cellular invasion and proliferation |
JAM-A | 4.5 | Activates Rap1 GTPase and upregulates β1-integrin | Increases cell motility Indicates poor patient prognosis |
PROTEINS UPREGULATED IN BONE-TROPIC MCF7-5624A CELLS | |||
Notch-1 | 2 | Activated by downstream products of TGFβ-SMAD pathway to promote osteoclast differentiation in bone | Promotes bone reabsorption and osteolytic lesions |
Amphiregulin | 2 | Stimulates and increases EGFR signaling | Important for bone colonization |
EGFR | 2.27 | Activation with MMP-1 leads to reduced production of osteoprotegrin, increasing RANKL activation of osteoclasts | Impacts recruitment of osteoblasts, limiting bone mineralization |