Table 2.
Cell Cycle Arrest. A) HT-29 and B) SW948 cells were exposed 24h to above treatment. The median of three independent experiments is shown. Eup= eupatorin and sal= salvigenin. *Significantly different from control cells (*P<0.05, **P<0.01, ***P<0.001) ¥ significantly different from eupatorin (¥P<0.05 and ¥¥¥P<0.001) and ψ significantly different from salvigenin (ψP<0.05).
| A | ||||||
|---|---|---|---|---|---|---|
| HT-29 cell line | control | Dox10µM | Eupatorin | Salvigenin | Dox(1µM)+Eup | Dox(1µM)+Sal |
| Freq. G1 | 53.37±4.25 | 51.39±3.66 | 39.33±4.12 | 63.07±3.12 | 39.55±2.98 | 43.74±3.47 |
| Freq. S | 18.55±2.36 | 26.59±2.01 | 20.01±2.18 | 26.84±2.84 | 20.23±2.19 | 22.39±2.55 |
| Freq. G2 | 25.29±1.69 | 13.93±1.22 | 39.19±2.02 | 16.27±1.08 | 18.75±1.11 | 27.14±1.97 |
| Freq.sub G1 | 1.88±0.12 | 11.98±0.95 ** | 4.25±0.38 * | 5.84±0.45 * | 20.03±1.02 *** ¥¥¥ | 9.99±0.97 ** ψ |
| B | ||||||
| SW-948 cell line | control | Dox10µM | Eupatorin | Salvigenin | Dox(1µM)+Eup | Dox(1µM)+Sal |
| Freq. G1 | 53.35±3.15 | 63.07±3.25 | 43.67±2.89 | 63.31±3.67 | 57.07±3.49 | 55.09±2.94 |
| Freq. S | 14.22±1.22 | 11.36±1.09 | 12.31±1.11 | 26.84±1.96 | 22.79±1.67 | 12.11±1.33 |
| Freq. G2 | 25.12±1.19 | 11.27±0.94 | 39.78±1.22 | 10.27±0.91 | 26.12±1.03 | 30.03±2.08 |
| Freq. sub G1 | 1.03±0.18 | 9.17±0.11 ** | 2.5±0.19 * | 4.71±0.33 ** | 5.34±0.37 ** ¥ | 5.84±0.39 ** ψ |