Table 3.
Apoptosis Percentage. The effect of eupatorin, salvigenin and doxorubicin on apoptosis in A) HT-29 and B) SW948. Flow cytometry detection of apoptosis with Annexin V/PI. The median of three independent experiments is shown. Eup= eupatorin and sal= salvigenin. *Significantly different from control cells (*P<0.05, **P<0.01, ***P<0.001), ¥ significantly different from eupatorin (¥P<0.05 and ¥¥P<0.01) and ψ significantly different from salvigenin (ψP<0.05 and ψψψP<0.001).
| A | ||||||
|---|---|---|---|---|---|---|
| HT-29 cell line | control | Dox | Eupatorin | Salvigenin | Dox(1µM)+Eup | Dox(1µM)+Sal |
| Necrosis | 3.37±0.18 | 18.33±1.17 ** | 5.63±0.26 * | 2.36±0.12 * | 7.30±0.84 * | 18.9±1.7 ** |
| late apoptosis | 0.297±.019 | 66.6±5.97 *** | 46.5±2.97 ** | 9.46±0.88 * | 83.2±5.36 ***, ¥¥ | 71.3±4.97 ***,ψψψ |
| Early apoptosis | 0.872±.028 | 5.31±0.49 ** | 4.80±0.36 ** | 13.80±1.02 *** | 3.16±0.28 ** | 3.18±0.24 **,ψ |
| Viable cells | 95.5±3.66 | 6.58±0.51 *** | 43±4.39 ** | 74.4±4.64 ** | 6.38±0.78 ***,¥¥ | 2.90±0.16 ***,ψψψ |
| B | ||||||
| SW948 cell line | control | Dox (10µM) | Eupatorin | Salvigenin | Dox(1µM)+Eup | Dox(1µM)+Sal |
| Necrosis | 0.869±0.059 | 16.33±1.89 ** | 4.44±0.36 * | 6.02±0.45 * | 11±0.92 **,¥ | 5.26±0.32 * |
| late apoptosis | 0.363±0.019 | 72.8±4.15 *** | 20.7±2.01 ** | 19.9±1.5 ** | 49.4±3.18 ***,¥¥ | 63.44±4.61 ***,ψψψ |
| Early apoptosis | 1.17±0.07 | 2.31±0.18 * | 27.1±2.08 *** | 20.9±1.32 ** | 19.6±0.89 **,¥ | 7.9±0.52 *,ψψψ |
| Viable cells | 97.6±5.19 | 6.58±0.37 *** | 44.4±2.87 ** | 48.36±3.02 ** | 16.19±0.98 *,¥¥ | 22.1±1.14 *,ψψψ |