PGV-1 Enhanced Cytotoxic Effect of 5-FU Toward WiDr Cells. Cells were treated with (A) 5-FU, curcumin, and PGV-1 at 24h of incubation; (B) 5-FU and (C) PGV-1 at various concentrations for indicated incubation times. (D) WiDr cells were treated with 5-FU in combination with PGV-1 at various concentration for 6, 12, 24, and 48 hours. MTT assays were performed to determined cell viability which is defined as (absorbance of treated cells-absorbance of blank)/(absorbance untreated cells-blank)x100%. The data represents mean + SD.