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Effect of PGV-1 and 5-FU on Cell Cycle Regulators. Cells were treated with various concentrations of (A) 5-FU and PGV-1 alone or (B) in combination for 24 hours. Cells were harvested and lysed with RIPA buffer to prepare the whole cell extracts. CBB protein assays were carried out to determine the protein concentration. (C) Quantification of western blot data using ImageJ software.
