Figure 10. Combined en >Hh;^HAHh^C85S expression restores ptc-LacZ expression in the wing disc.

(a) Ptc-LacZ reporter gene expression at the a/p border in wild-type third-instar discs. Nuclear β-galactosidase is immunofluorescently labeled (red). 4D9 α-engrailed/invected (inv) antibodies label the posterior compartment in this and the following panels (green). The left image is a merge. Fly larvae developed at 25°C. (b) En-controlled Hh overexpression increased and expanded ptc-LacZ expression anteriorly. (c) En-controlled ^HAHh^C85S overexpression reduced ptc-LacZ expression, as previously shown. (d) En-controlled Hh;^HAHh^C85S coexpression generated a stripe of intense expanded ptc-LacZ staining, consistent with the gain-of-function phenotype observed in wings. Wing discs are oriented such that anterior is right and dorsal is up; all magnification, camera and processor settings were kept identical. Scale bar: 100 μm.