Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Drosophila melanogaster) | Hedgehog; Hh | PMID 8252628 | NCBI Reference sequence: NM_001038976.1 |
|
| Cell line (D. melanogaster) |
Schneider 2 | Invitrogen | RRID: CVCL_Z232 | |
| Cell line (Homo sapiens) | Bosc23 | PMID: 11395778 | RRID: CVCL_4401 | |
| Transfected construct (Hedgehog) |
Hh | PMID 8252628 | NCBI Reference sequence: NM_001038976 |
|
| Transfected construct (Sonic hedgehog) |
Shh | PMID: 7916661 | NCBI Reference sequence: NM_009170 |
|
| Antibody | anti-Hh | Santa Cruz | d300 catalog # sc-25759 | 2000-fold at 4°C over night |
| Antibody | anti-Shh | R and D Systems | Catalog # AF464 | 1000-fold at 4°C over night |
| Antibody | anti-en | DSHB | DSHB # 4D9 | 50-fold at 4°C over night |
| Antibody | anti-LacZ | Cappel, MP Biomedicals | Catalog # 08559761 | 50-fold at 4°C over night |
| Antibody | anti-HA | Sigma | catalog # H9658 | 5000-fold at 4°C over night |
| Strain, strain background (D. melanogaster) |
Ptc > Gal4 | Bloomington # 2017 | ||
| Strain, strain background (D. melanogaster) |
En > Gal4 | FlyBaseID FBrf0098595 | ||
| Strain, strain background (D. melanogaster) |
Hh > Gal4 | Bloomington # 67046 | ||
| Strain, strain background (D. melanogaster) |
34B > Gal4 | Bloomington # 1967 |