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(B, C, E and F) Western blotting using LC3A/B-II/I, p62, ATG-5 and ATG-7 antibodies on cryopreserved valve cells treated with trehalose (0.1 mol/L) or DMSO for 16 weeks. Data are expressed as the mean ± SD (n = 20 per group). **P < 0.01, ***P < 0.001 versus the DMSO group.
