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. 2018 Jan 12;9(15):11905–11921. doi: 10.18632/oncotarget.24190

Figure 7. Effects of chloroquine on testosterone-treated C2C12 cells.

Figure 7

Differentiated C2C12 cells were pre-treated with chloroquine (0, 30 μM) for 2 h, followed by the treatment with T (0, 5 × 10-7 M) for 24 h. (A) Representative Western blots and densitometry quantification of (B) LC3-II/LC3-I ratio, and (C) p62. EIF-5 was used as a control. (D) Ratio of integrated mitochondrial to nuclear DNA number. (E) Normalized ATP production. (F) Representative Western blots and densitometry quantification of plasma membrane GLUT4. Na/K-ATPase served as a loading control of plasma membrane protein. (G) 2-NBDG glucose uptake assay. (H) Insulin-signaling assay. Data are expressed as mean ± SEM. *P < 0.05, **P < 0.01, vs control; #P < 0.05, ##P < 0.01. n = 6/group. T: testosterone, CQ: chloroquine. GLUT4: glucose transporter 4.

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