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. 2018 Jan 10;9(15):12020–12034. doi: 10.18632/oncotarget.24147

Figure 3. Autophagy is enhanced in the gefitinib-resistant NSCLC cells and tissues.

Figure 3

(A) WB detection of LC3B-I, LC3B-II, and P62 proteins in PC9 and PC9/GR cells (left panel) and HCC827 and HCC827/GR cells (right panel). Actin and GAPDH served as loading controls. (B) TEM images of PC9 and PC9/GR cells (left panel) and HCC827 and HCC827/GR cells (right panel). Red arrows point to autophagic vacuoles presented in gefitinib-resistant cells (PC9/GR and HCC827/GR), which are absent in gefitinib-sensitive cells (PC9 and HCC827). (C) Confocal microscopic images of the lysosomes in the PC9 and PC9/GR cells (left panel) and HCC827 and HCC827/GR cells (right panel). Red: lysosome tracker-stained lysosome. Blue: Hoechst 33258-stained nuclei. The green arrow points to the lysosome. (D) Immunohistochemical staining of Ki-67 and LC3B proteins in xenograft tumor tissues derived from PC9 (lane 1), PC9/GR (lane 2), HCC827 (lane 3), and HCC827/GR cells (lane 4).