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. 2018 Jan 10;9(15):12020–12034. doi: 10.18632/oncotarget.24147

Figure 4. Inhibition of autophagy suppresses gefitinib resistance.

Figure 4

(A) PC9/GR and HCC827/GR cells were treated with either 0 or 100 µM 3-MA. (B) PC9/GR and HCC827/GR cells were treated with 0, 10, or 20 µM CQ for 2 days in combination with either DMSO (as the control) or 3 µM gefitinib, and then continued to proliferate and form colonies for 12 days. (C) WB validation of ATG5 knockdown by three different siRNA against ATG5 (si-ATG5#1, #2, or #3), compared with the control siRNA (si-Control) in PC9/GR and HCC827/GR cells. (D) Colony formation assay of PC9/GR and HCC827/GR cells transfected with si-ATG5#1, #2 or #3, compared with si-Control. (E) The relative tumor growth curves of PC9/GR derived xenograft tumors under different treatments. (F) Mice body weight curve over the same period of time as in (E).