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. 2018 Jan 3;9(15):12050–12063. doi: 10.18632/oncotarget.23927

Figure 7. Mix5-10-11 multi-subunit vaccine elicited stronger Th1 and Th17 responses to protect against H. pylori infection.

Figure 7

Th1 and Th17 responses were evaluated in both multi-subunit vaccine Mix5-10-11 immunized mice and Mix5-10-11-specific CD4 T cell-adapted mice. In the Mix5-10-11, PC05, or PBS immunized/challenged groups, (A) mRNA levels of INF-γ and IL-17A were evaluated in isolated stomach lymphocytes with real-time PCR, N = 10. Meanwhile, spleen lymphocytes were isolated from the Mix5-10-11 or PC05 immunized mice and cultured with PC05 to obtain PC05-specific CD4 T cells. Then, the PC05-specific CD4 T cells from each group were stimulated with P5, P10, or P11. P5-, P10-, and P11-specific Th1 (B) and Th17 (C) responses were detected by flow cytometry, N = 5. (D) Th1 and Th17 responses were monitored in mice injected with Mix5-10-11 or PC05-specific CD4 T cells. Lymphocytes were isolated from the spleens of the Mix5-10-11 or PC05-specific CD4 T cell adapted mice and stimulated with Mix5-10-11 or PC05 respectively. Th1 and Th17 responses were monitored before H. pylori challenge to ensure that the transferred Mix5-10-11 or PC05-specific Th1 and Th17 cells were active. One-way ANOVA test with post-test Bonferoni. **P < 0.01, ***P < 0.001.