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. 2018 Jan 6;9(15):12174–12185. doi: 10.18632/oncotarget.24040

Figure 4. High levels of CD163 expression determine PRRSV replication.

Figure 4

(A) Three CD163 expression patterns (low, middle and high levels) were isolated using flow cytometry sorting technology. The representative isolated cell clones were designated PAM-Tang-low, PAM-Tang-middle and PAM-Tang-high, respectively. (B) The CD163 expression levels of PAM-Tang-low, PAM-Tang-middle and PAM-Tang-high cells were confirmed by flow cytometry and (C) Western blotting. (D) PAM-Tang, PAM-Tang-low, PAM-Tang-middle and PAM-Tang-high cells showed different susceptibilities to PRRSV infection. All immortalized cells were infected with PRRSV HuN4 at an MOI of 1. Five days post-infection, the infected cells were examined under an inverted fluorescence microscope and by (E) flow cytometry and (F) Western blotting. The above experiments were performed three times, and a representative result is shown for each. (G, H and I) Different cell lines were infected with different MOIs (1, 0.1 and 0.01), and 72 h post-infection, their viral titers were determined by real-time PCR.