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. 2018 Jan 6;9(15):12174–12185. doi: 10.18632/oncotarget.24040

Figure 5. CD163 is critical for PRRSV replication.

Figure 5

(A) CD 163-negative cells isolated from PAM-Tang-high cells by flow cytometry. (B) CD163 depletion in PAM-Tang-high sufficiently blocks PRRSV replication. (C) PRV infection was not influenced by CD163 abundance. A firefly luciferase-tagged PRV (MOI = 1) was utilized to infect different cell lines, and luciferase activity was evaluated at 24 h post-infection. (D) PRRSV attachment was not influenced by CD163 abundance. (E) The CD163 level of PAM-Tang-high cells did not significantly change during the course of 10 passages. (F) Cellular susceptibility to PRRSV did not significantly change during the course of 10 passages. PAM-Tang-high P1 and PAM-Tang-high P10 were infected with 1 MOI of PRRSV HuN4, and their viral titers were determined by RT-PCR at 72 h post-infection.