Figure 3.

(A) SUM159 cells transfected and selected for stable expression of ERβ2 and ERβ5. Real time PCR was used to analyze changes in signaling pathways (basal cell factors, proliferative factors, EMT inducing and stem cell associated factors as well as NF-kB signaling). (B) 500 cells of each SUM159 ERβ2 and ERβ5 were plated on each well in a 6 well plate in media containing 2% serum and incubated for 4 days; the cells were visualized with crystal violet staining. (C) Western blot of Control, ERβ2 and ERβ5 expressing SUM159 cells exposed to normoxia or hypoxia for 4 hours at 1% O_2, detection by HIF-1α antibody.