Figure 1.
A. Transmission paradigm. Donor fish with high levels of intestinal tumors were co-housed with healthy casper fish (Exposures A and B). After exposure to donors, transmission of disease was propagated by exposing healthy 5D fish to effluent from tanks of Exposure B fish (Exposures C and D). After Exposure C was exposed to Exposure B, Exposure C fish were co-housed with healthy Casper fish (Exposure E). Red arrows indicate transmission of pathology from one group to another. B. Incidence of intestinal lesions in donor and recipient zebrafish. Preneoplastic designates hyperplasia or dysplasia of epithelium. All fish with neoplasia had preneoplastic lesions in other regions of the intestine, but these fish were not included in the “preneoplastic” data. M = moribund fish. PCR + or – indicates samples positive or negative Mycoplasma PCR test.