Fig. 4.

FOXO3a, a downstream target of AMPK, inhibits GOF mutant p53-mediated FOXM1 expression in HNSCC cells. a Ingenuity Pathway Analysis of possible upstream transcription factors regulating FOXM1 expression. b Western blot analysis of CRISPR-Cas9/FOXO3a KO UM-SCC-1 cell lines in the presence or absence of metabolic stress under the confluent high-density culture condition. c Western blot analysis of isogenic UM-SCC-1 stable cell lines in the presence or absence of metabolic stress under the confluent high-density culture condition. pBMN-EV, empty vector. d Real-time RT-qPCR analysis of mRNA transcripts of FOXM1b and -1c from UM-SCC-1 stable cells under the confluent high-density culture condition. * <0.05; **p < 0.01. pLVX-EV, empty vector. e Western blot analysis of Detroit 562 stable cell lines in the presence or absence of metabolic stress under the confluent high-density culture condition. f Real-time RT-qPCR analysis of FOXM1b mRNA transcripts from Detroit 562 stable cells in the presence or absence of metabolic stress under the confluent high-density culture condition. *<0.05; **p < 0.01