Sorted blood ILC2s were cultured with 10 ng/ml IL-33 and 10 ng/ml TSLP in the presence or absence of 10 ng/ml IL-10 (B, n=8), 20 ng/ml TGF-β1 (C, n=6), 0.01% DMSO (vehicle control) and 100 nM dexamethasone (Dex) (D, n=6) for 4 days. The morphology of ILC2s was examined by cytospin with Diff-Quik staining (A). The concentrations of IL-4, IL-5, IL-9 and IL-13 were measured by using Luminex (B–D). * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001, by one-way ANOVA.