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. Author manuscript; available in PMC: 2018 Jun 18.
Published in final edited form as: Oncogene. 2017 Dec 18;37(10):1308–1325. doi: 10.1038/s41388-017-0023-0

Figure 6. ER-Golgi trafficking genes promote metastatic progression.

Figure 6

(a) Tumor volume of mammary fat pad injected L2P cells stably expressing scrambled control, ARF4, COPB1 and USO1 shRNA (n = 5 animals for control, ARF4 KD and COPB1 KD groups, n = 4 animals for USO1 KD group). (b) Image of excised tumors and (c) quantitation of tumor weight from mice that were mammary fat pad injected with L2P cells stably expressing scrambled control, ARF4, COPB1 and USO1 shRNA (data not significant; unpaired t-test, scale bar: 1 cm). (d) Incidence of lung metastasis in mice following mammary fat pad injection of control, ARF4, COPB1 and USO1 KD L2P cells. (e) Representative images of H&E and Ki67 stained lung tissue sections following tail vein injection of control, ARF4, COPB1 and USO1 KD L2P cells. Scale bar: 100 µm (H&E) and 50 µm (Ki67). (f) Incidence of lung metastasis, (g) Tumor/lung ratio and (h) Ki67 positivity in lungs of mice tail vein injected with control, ARF4, COPB1 and USO1 KD L2P cells (n = 9 animals for control and USO1 KD groups, n = 8 animals for ARF4 KD and COPB1 KD groups, **P < 0.01; unpaired t-test).