. 2018 Mar 9;8:4232. doi: 10.1038/s41598-018-22708-9

Table 1.

Sensitivity and specificity of tests measuring antibodies to Pgp3 based on input parameters. Pos = positive; ind = indeterminate; neg = negative. LFA = lateral flow assay; ELISA = enzyme-linked immunosorbent assay; MBA = multiplex bead array. ROC = receiver operator characteristics; MM = mixture model. DF = degrees of freedom. AIC = Akaike Information Criterion. BIC = Bayesian Information Criterion. Bolded rows are referenced in the text.

Model #	Latent Classes	Diagnosis Categories	LFA Blood test	LFA Serum test	ELISA coating	Cutoff	N	DF	AIC	BIC	G² test p-value	χ² test p-value	Proportion positive	MBA Sensitivity	MBA Specificity	ELISA Sensitivity	ELISA Specificity	LFA Sensitivity	LFA Specificity
1	2	Pos/neg	Yes	Yes	Fresh	ROC	283	4	743.6	783.7	0.11	< 0.01	0.36 (0.28–0.44)	0.95 (0.87–1.00)	0.98 (0.96–1.00)	0.87 (0.77–0.96)	0.98 (0.94–1.00)	0.87 (0.77–0.97)	0.96 (0.91–1.00)
2	2	Pos/neg	Yes	Yes	Dried	ROC	283	4	736.6	776.7	< 0.01	< 0.01	0.35 (0.27–0.43)	0.96 (0.90–1.00)	0.98 (0.95–1.00)	0.81 (0.69–0.92)	0.99 (0.97–1.00)	0.88 (0.79–0.98)	0.95 (0.90–1.00)
3	2	Pos/neg	Yes	Yes	Fresh	MM	283	4	742.2	782.2	0.08	0.01	0.37 (0.29–0.45)	0.92 (0.84–1.00)	0.99 (0.96–1.00)	0.99 (0.96–1.00)	0.91 (0.85–0.98)	0.85 (0.75–0.95)	0.97 (0.93–1.00)
4	2	Pos/neg	Yes	Yes	Dried	MM	283	4	692.1	732.2	0.14	0.03	0.37 (0.29–0.45)	0.93 (0.85–1.00)	0.98 (0.96–1.00)	0.99 (0.96–1.00)	0.96 (0.92–1.00)	0.86 (0.76–0.96)	0.97 (0.93–1.00)
5	2	Pos/neg	No	Yes	Fresh	ROC	569	0	1247.1	1277.5			0.42	0.97	0.98	0.89	0.98	0.99	0.97
6	2	Pos/neg	No	Yes	Dried	ROC	569	0	1266.8	1297.2			0.41	0.98	0.98	0.86	0.98	0.99	0.96
7	2	Pos/neg	No	Yes	Fresh	MM	569	0	1210.2	1240.6			0.43	0.95	0.99	1.00	0.92	0.98	0.98
8	2	Pos/neg	No	Yes	Dried	MM	569	0	1144.8	1175.2			0.43	0.95	0.98	1.00	0.96	0.99	0.98
9	2	Pos/neg	Yes	No	Fresh	ROC	289	0	675.3	701.0			0.34	0.96	0.97	0.87	0.96	0.91	0.99
10	2	Pos/neg	Yes	No	Dried	ROC	289	0	647.8	673.5			0.33	0.96	0.96	0.84	0.99	0.94	0.99
11	2	Pos/neg	Yes	No	Fresh	MM	289	0	676.3	702.0			0.36	0.95	0.99	0.99	0.89	0.88	0.99
12	2	Pos/neg	Yes	No	Dried	MM	289	0	633.0	658.7			0.35	0.95	0.98	0.99	0.95	0.90	0.99
13	2	Pos/ind/neg	Yes	Yes	Fresh	ROC	297	48	1102.9	1187.9	0.15	< 0.01	0.39 (0.33–0.44)	0.89 (0.82–0.95)	0.97 (0.94–0.99)	0.70 (0.60–0.79)	0.97 (0.95–1.00)	0.77 (0.69–0.86)	0.96 (0.92–0.99)
14	2	Pos/ind/neg	No	Yes	Fresh	ROC	579	13	1632.2	1688.9	< 0.01	< 0.01	0.42 (0.38–0.47)	0.95 (0.91–0.98)	0.97 (0.95–0.99)	0.80 (0.74–0.86)	0.96 (0.94–0.99)	0.97 (0.94–0.99)	0.96 (0.94–0.99)
15	2	Pos/ind/neg	Yes	No	Fresh	ROC	297	13	956.0	1004	< 0.01	< 0.01	0.39 (0.32–0.45)	0.89 (0.81–0.96)	0.96 (0.93–1.00)	0.70 (0.59–0.80)	0.97 (0.94–1.00)	0.80 (0.71–0.89)	1.00 (0.98–1.00)
16	3	Pos/ind/neg	Yes	Yes	Fresh	ROC	297	36	1096.4	1225.7	0.84	0.04	0.27 (0.11–0.43)	0.99 (0.91–1.00)	0.97 (0.94–1.00)	0.92 (0.63–1.00)	0.98 (0.95–1.00)	0.89 (0.79–0.99)	0.96 (0.93–0.99)
17	3	Pos/ind/neg	No	Yes	Fresh	ROC	579	6	1613.4	1700.6	0.85	0.95	0.38 (0.30–0.46)	0.98 (0.96–1.00)	0.98 (0.96–1.00)	0.90 (0.73–1.00)	0.97 (0.95–0.99)	0.98 (0.96–1.00)	0.96 (0.94–0.99)
18	3	Pos/ind/neg	Yes	No	Fresh	ROC	297	6	939.5	1013.4	0.09	0.04	0.35 (0.27–0.42)	0.95 (0.89–1.00)	0.99 (0.96–1.00)	0.78 (0.65–0.91)	0.98 (0.94–1.00)	0.84 (0.75–0.94)	1.00 (0.98–1.00)
19	2	Pos/ind/neg	Yes	Yes	Dried	ROC	297	48	1129.1	1214.1	0.03	< 0.01	0.39 (0.33–0.44)	0.89 (0.82–0.96)	0.96 (0.94–0.99)	0.61 (0.51–0.72)	0.97 (0.94–1.00)	0.78 (0.70–0.86)	0.95 (0.92–0.99)
20	2	Pos/ind/neg	No	Yes	Dried	ROC	579	13	1675.4	1732.1	< 0.01	< 0.01	0.42 (0.38–0.47)	0.95 (0.92–0.99)	0.97 (0.95–0.99)	0.75 (0.69–0.82)	0.97 (0.94–0.99)	0.97 (0.94–1.00)	0.96 (0.93–0.98)
21	2	Pos/ind/neg	Yes	No	Dried	ROC	297	13	976.4	1024.4	< 0.01	< 0.01	0.37 (0.31–0.43)	0.90 (0.82–0.97)	0.94 (0.90–0.99)	0.63 (0.52–0.75)	0.96 (0.93–1.00)	0.84 (0.75–0.92)	1.00 (0.98–1.00)
22	3	Pos/ind/neg	Yes	Yes	Dried	ROC	297	36	1109.8	1239.1	0.92	0.93	0.27 (0.18–0.37)	0.99 (0.96–1.00)	0.97 (0.94–1.00)	0.86 (0.59–1.00)	0.98 (0.95–1.00)	0.89 (0.82–0.96)	0.96 (0.93–0.99)
23	3	Pos/ind/neg	No	Yes	Dried	ROC	579	6	1650.0	1737.2	0.45	0.62	0.37 (0.27–0.47)	0.99 (0.98–1.00)	0.98 (0.96–1.00)	0.86 (0.66–1.00)	0.97 (0.95–1.00)	0.98 (0.96–1.00)	0.97 (0.93–1.00)
24	3	Pos/ind/neg	Yes	No	Dried	ROC	297	6	951.8	1025.7	0.09	0.17	0.30 (0.21–0.38)	0.98 (0.95–1.00)	0.99 (0.93–1.00)	0.79 (0.60–0.98)	0.99 (0.95–1.00)	0.90 (0.82–0.99)	1.00 (1.00–1.00)