Table 1.

Anti-proliferative activity of the benzofurane derivatives in endothelial and tumor cell lines and binding constants for αβ-tubulin.

Comp.	Tumor cells IC50 (μM)a			Endothelial cells IC50 (μM)a	Kb (M−1)
	MDA-MB-231	CEM	HeLa	HMEC-1
8	0.068 ± 0.005	0.19 ± 0.01	0.18 ± 0.0	0.10 ± 0.02	(13 ± 2) × 106(b)
14	0.053 ± 0.006	0.038 ± 0.001	0.16 ± 0.01	0.065 ± 0.003	(89.5 ± 3.6) × 106(b)
20	0.17 ± 0.01	0.64 ± 0.36	0.89 ± 0.03	1.6 ± 0.6	(1.57 ± 0.35) × 106(b)
21	1.5 ± 0.6	5.5 ± 1.7	12 ± 6	14 ± 11	(0.07 ± 0.01) × 106(b)
22	0.015 ± 0.002	0.040 ± 0.002	0.14 ± 0.04	0.15 ± 0.05	(91.0 ± 10.1) × 106(b)
27	0.009 ± 0.001	0.031 ± 0.002	0.030 ± 0.002	0.016 ± 0.002	(287 ± 106) × 106(b)
28	0.012 ± 0.005	0.051 ± 0.018	0.034 ± 0.003	0.038 ± 0.003	(65.1 ± 6.80) × 106(b)
Colchicine	0.007 ± 0.003	0.013 ± 0.0004	0.0087 ± 0.0001	0.0038 ± 0.0011	11.6 × 106(c)63
CA-4P	0.0021 ± 0.0003	0.011 ± 0.001	0.013 ± 0.001	0.0029 ± 0.0001
Podophillotoxin	—	—	—	—	1.8 × 106 64
R-PT	—	—	—	—	3.2 × 106 46

^aIC₅₀ (50% inhibitory concentration) is given as the mean ± SD of three independent experiments.

^bMean value of three experiments ± SD.

^cAt 37 °C.