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. 2018 Mar 9;8:4286. doi: 10.1038/s41598-018-22644-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of NPs with one square micron AFM maps of SPION nanoparticles. Figures in the left and right represent the height and phase images, respectively. The dextran coated particles (PDS0) are forming large aggregates as well as individual NPs, and PEGylation leads to less large-scale aggregation, and smaller-scale clusters. The topography maps for PDS1 and PDS8 show the formation of complex aggregates of NPs where individual particles are connected by PEG.