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. 2018 Mar 9;8:4286. doi: 10.1038/s41598-018-22644-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Biodistribution and blood retention analyses 24 h post injection. (a) 40 μm tissue sections from different organs were stained by Prussian blue. Control group was injected with PBS. Dark blue spots indicate the presence of Fe. Note that natural ferric ions exist in spleen. Figures are taken by 10X magnification. Image quantification results for (b) liver and (c) spleen. (n = 3) (d) Blood retentions of samples over 24-h post injection. All the mice were administered intravenously with 2 mg/mL dose of NPs. Data represent mean ± SD (n = 3) of injected dose percentage against time. Statistical analysis was assessed against PDS0.