Table 2.

Bootstrap support cutoff values that produced at least 99% correct assignments during CO1 Eukaryote v1 leave-one-out testing.

Rank	500 bp+	400 bp	200 bp	100 bp	50 bp
	Minimum bootstrap support cutoff (%)
Superkingdom	0	0	0	0	0
Kingdom	0	0	0	0	0
Phylum	0	0	0	0	0
Class	0	0	0	0	60
Order	0	0	10	40	80
Family	20	20	30	40	80
Genus	70	60	60	60	N/A
	Reduction of sequences classified after applying minimum bootstrap support cutoff (%)
Superkingdom	0.0	0.0	0.0	0.0	0.0
Kingdom	0.0	0.0	0.0	0.0	0.0
Phylum	0.0	0.0	0.0	0.0	0.0
Class	0.0	0.0	0.0	0.0	13.2
Order	0.0	0.0	0.2	5.7	60.7
Family	0.7	1.2	4.1	17.1	78.7
Genus	3.4	4.7	10.8	31.0	N/A

‘N/A’, not applicable, refers to the inability to observe 99% correct taxonomic assignments.