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. 2018 Mar 9;8:4248. doi: 10.1038/s41598-018-22557-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HDM induces lung inflammation. (A) Differential cell count in BALF (B) Total protein (C) Release of the pan-necrotic marker LDH in BALF (D) H&E staining of mice lung sections (E) Inflammation scored in lung tissue. (F) Representative immunoblots of cleaved caspase-3 and cleaved PARP. (G) Quantification of protein expression of cleaved caspase-3 and (H) cleaved PARP from immunoblots. Optical density was measured and bands related to housekeeping protein GAPDH and normalized towards saline. The data are presented as mean ± SEM (n = 5–6). *p < 0.05 vs saline, **p < 0.01 vs saline.