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. 2018 Mar 9;8:4309. doi: 10.1038/s41598-018-22342-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Satellite cell number and myofiber size are normal in un-injured Ts65Dn muscle. (A) Un-injured TA muscle sections stained with anti-Pax7 antibody to label satellite cells (red) and laminin (green) to label the basal lamina. Blue is DAPI. White carets mark satellite cells. (B) Myofibers isolated from EDL muscle were fixed immediately and stained with anti-Pax7 antibody to identify satellite cells. Blue is DAPI. White carets mark satellite cells. (C–E) Quantification of Pax7+ satellite cell number and average fiber size in Ts65Dn muscle compared to wild type (n = 3 or 4). Statistical significance was determined using Student’s t test using. P-value < 0.05 were considered significant. NS indicates not significant Scale bars are 40 μm.