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. 2018 Mar 9;8:4309. doi: 10.1038/s41598-018-22342-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Reduced number of Ts65Dn satellite cells at 4 days post injury. (A) Images of TA muscle collected 4 days post injury were visualized for Pax7 immunoreactivity (red) to label satellite cells, laminin immunoreactiviey to mark myofibers (green) and EdU (yellow). EdU was injected 2 h prior to collection. Blue is DAPI. White carets mark EdU+ satellite cells. (B) Quantification of satellite cell numbers at 4 days post injury in Ts65Dn muscle and wild type muscle (n = 3). (C) Quantification showing the percentage of Pax7+/EdU+ cells in wild type TA muscle and Ts65Dn TA muscle at 4 day post injury (n = 3). Statistical significance was tested using Student’s t test. Asterisks indicates significant difference and P-value < 0.05. NS indicates not significant. Scale bar is 40 μm.