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. 2018 Mar 9;8:4309. doi: 10.1038/s41598-018-22342-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Increased Usp16 expression and DNA damage in Ts65Dn satellite cells. (A) Semi-quantitative RT-PCR analysis for Usp16 amplification products from cDNA of Ts65Dn satellite cells compared to wild type satellite cells. The images shown were cropped to save space (full-length gel included in Supplementary Information). (B) Quantitative real-time PCR for Usp16 expression in Ts65Dn satellite cells compared to wild type satellite cells (n = 3). (C) Images for isolated myofibers cultured for 24 h immunoreactive for γH2AX (green, DNA damage) and immunoreactive for Syndecan-4 (red, satellite cells). Blue is DAPI. (D) Quantification of DNA damage foci identified by γH2AX labeling in Ts65Dn satellite cells and wild type satellite cells (n = 3). Statistical significance was tested using Student’s t test. Asterisks indicates significant difference and P-value < 0.05. Scale bar is 10 μm.