Figure 5.

Addition of exogenous cholesterol increased cannabidiol-induced TRPV2 calcium influx. (A) Confocal images with the raft marker dye, CTX-B, which recognize the GM1 ganglioside localization in human primary macrophages after cholesterol treatment (15 µg/ml). Images are representative of five separate experiments. Data representative of the GM1 fluorescence are shown below as mean ± s.e.m. Each point are representative of the fluorescence quantification of at least 30 cells coming from at least five fields. Mann-Whitney test: *p < 0.05 vs. control. (B) Fura-2 AM calcium measurement was performed in human macrophages stimulated by cannabidiol (75 µM) in the absence (control) or presence of cholesterol (15 µg/ml, 30 min pretreatment). Data are presented as the ratio of emission after excitation at 340 nm relative to that after excitation at 380 nm (F340/F380) and normalized to basal level 1. Horizontal bar represented stimulus period. Data are representative of three independent experiments. Below, area under the curve of similar experiments are shown as mean ± s.e.m. Mann Whitney test: *p < 0.05 vs. control.