Figure 7.

CFTR inhibition decreased TRPV2 mediated-Ca²⁺ influx. (A) TRPV2 gene expression in macrophages treated or not with CFTR_inh-172 (10 µM, 72 h pretreatment) (n = 6). TRPV2 mRNA expression was determined by RT-qPCR. (B) Representative blot of TRPV2 (110 kDa) protein level in total cellular lysate from human macrophage in the absence (control) or presence of CFTR_inh-172 (10 µM, 72 h pretreatment). Equal protein loading was controlled via HSC70 detection. Immunoblots are representative of four independent experiments. Full-length blots are presented in Supplementary Figure 3. The scatter dot plot represents densitometric analysis. Results are shown as the mean ± s.e.m. (C) Fura-2 AM calcium measurement in human macrophages cells stimulated by cannabidiol (75 µM) in the absence (control) or presence of CFTR_inh-172 (10 µM, 72 h pretreatment). Tranilast (100 µM) is used as control for inhibition of TRPV2 mediated-Ca²⁺ influx. Data are given as the ratio of emission after excitation at 340 nm relative to that after excitation at 380 nm (F340/F380), and normalized to basal level 1. Data are representative of three independent experiments. Below, area under the curve of similar experiments are shown as mean ± s.e.m. Mann Whitney test.