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. 2018 Mar 9;8:4233. doi: 10.1038/s41598-018-22699-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phylogenetic relationships of ehxA sequences based on the neighbour-joining method. Thirty-six unique ehxA sequences were obtained in this study. The serogroups for each ehxA genotype (representative strain) are given. Twenty-two sequences of six ehxA PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) subtypes A to F downloaded from GenBank are indicated in bold. Bootstrap values > 50% are shown at the branch points.