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. 2018 Mar 9;9:1025. doi: 10.1038/s41467-018-03343-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — The dLGN^TPR:Insc^ASYM complex adopts two conformations. a Superposition of the two dLGN^TPR:Insc^ASYM conformers present in the a.s.u. The most compact conformer is shown as ribbon diagram, and the most extended is depicted in transparent cylindrical helices. Subunits are colored as in Fig. 1. b, c Zoomed views of the interfaces between the helical bundle of Insc-A and TPR7-8 of dLGN-B for the compact (top) and extended (bottom) tetramers. The rearrangement of the TPR repeats of dLGN-B in the compact conformation is accompanied by disruption of the initial turns of helix α5 of Insc-A. d Graphical summary of the conformational changes between the extended and the compact tetramers. e SAXS data for the dLGN^TPR:Insc^ASYM complex in comparison to the theoretical curves for the different configurations (upper part and inlay) and fits of ab initio models in P1 (gray) and P2 symmetry (blue). Idealized curves were obtained merging frames with matching radius of gyration and high similarity according to CORMAP^38,39. f The ab initio model of the dLGN^TPR:Insc^ASYM complex in P1 symmetry (transparent gray spheres) matches well with both the extended (orange) and compact (green) tetramers