Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Mar 9;9:1025. doi: 10.1038/s41467-018-03343-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — Conserved organizational principles of Par3:Insc:LGN:Gαi complexes. a Reconstitution of human Par3:Insc:LGN interaction in cells. HEK293T cells were transfected with Par3-GFP, FLAG-LGN and HA-Insc. Cell lysates were subjected to IP with α-FLAG antibody conjugated to sepharose beads. After washes, species on beads were analyzed by SDS–PAGE and immunoblotted as indicated in the figure. HA-empty vector was included as a negative control of the IP. b SEC elution profiles and SDS–PAGE separation of Insc:LGN:Gαi complexes reconstituted from expression in insect cells (dark green trace) and Par3^PDZ123 purified from bacterial sources (pink trace). When the Par3^PDZ123 is mixed in molar excess with Insc:LGN:Gαi, it coelutes with Insc:LGN:Gαi in a stoichiometry reflecting the amount of Insc in the complex (light green trace). c Summary of the interactions in the Par3:Insc:LGN:Gαi complex supported by our study. Stable Insc:LGN tetramers form the core of apically localized Par3:Insc:LGN:Gαi complexes, in which the C-terminal portion of the two Insc chains contacts directly the PDZ domains of Par3. Binding of each LGN-GoLoco to four Gαi^GDP molecules stabilizes the association of the macromolecular assemblies to the plasma membrane