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. 2018 Mar 9;8:4305. doi: 10.1038/s41598-018-22364-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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B49Mod1 completely retains the anti-adhesion function of B49 without affecting cell viability. (A) Cartoon depiction a BST-2-targeting peptide (B49) and a smaller B49 analog that lacks penetratin at the N-terminus (B49Mod1) and is more stable as defined by half-life in silico analyses. (B,C) Cellular viability of several (B) human and (C) mouse breast cancer cell lines following a 24-hour treatment with vehicle (1:8 (v/v) acetonitrile:water) or with 200 ng/well of B49Mod1. (D) Adherence of E0771 shControl (shCTL) cells to E0771 shControl monolayers that were pre-treated with Vehicle, B49, or B49Mod1 for 4 hours at 200 ng/well before addition of incoming cells. (E,F) Adherence of PKH67-labeled E0771 shControl or shBST-2 cells to E0771 shControl monolayers that were pre-treated with (E) B49Mod1 at 200 ng/well or (F) with an equivalent volume of Vehicle for 4 hours. (G,H) Adherence of 4T1 (G) shControl or (H) shBST-2 cells to 4T1 shControl monolayers that were pre-treated with Vehicle (green) or B49Mod1 (gray) at 200 ng/well for 4 hours. (I,J) Adherence of 4T1 (I) shControl or (J) shBST-2 cells to 4T1 shBST-2 monolayers that were pre-treated with Vehicle (green) or B49Mod1 (gray) at 200 ng/well for 4 hours. Error bars correspond to SEM. Significance was taken at *P < 0.05, **P < 0.01 and ***P < 0.001. ns = not significant.