Fig. 2. Effects of inhibition of candidate proteases on Dsg2 cleavage.

a Western blot using an antibody against Dsg2 C-term and also for cleaved Notch of cell lysates of T-84s treated with TNF-α and IFN-γ for 24 h in the presence or absence of the γ-secretase inhibitor DAPT. b Western blot using an antibody against Dsg2 C-term of cell lysates of T-84s treated with TNF-α and IFN-γ in the presence or absence of increasing concentrations of calpeptin for 24 h. c Western blot using an antibody against Dsg2 N-term of cell culture supernatants of T-84s treated as in Fig. 2b. d Western blot for caspase-8 cell lysates of T-84s treated as in Fig. 2b. All blots are representative of at least three independent experiments