Fig. 5. Exogenous expression of Myc-tagged Dsg2 ICF sensitizes cells to apoptosis through downregulation of anti-apoptotic Bcl-2 family members.
a Model of the protein products of the adenoviral expression constructs used in this paper. b Western blot of cell lysates from SKCO15s expressing the indicated adenoviral expression constructs. Left. Western blot for the Myc tag. Right. Western blot using an antibody against Dsg2 C-term. c Left. Western blot for PARP, Bcl-XL, and Mcl1 of cell lysates from SKCO15s treated as in Fig. 5b. Right. Densitometry of blots for Bcl-XL and Mcl1. *p ≤ 0.05 n = 3 per group. For Bcl-XL p = 0.0003 and for Mcl1 p = 0.0017. d Left. Western blot for cytochrome c, GAPDH, and Bcl-2 of cytoplasmic fractions from SKCO15s treated as in Fig. 5b. Right. Densitometry of blots for cytochrome c normalized to GAPDH control. (For similar analysis of mitochondria containing fractions, see Supplemental Fig. 4B.) *p = 0.0093 n = 3 per group. All blots are representative of at least three independent experiments. Mito mitochondria, Cyto-c cytochrome c