Table 1.
Immunoreactive proteins of M. bovis planktonic cells identified by MALDI-TOF/TOF MS.
| Spot no. | Protein | Homology gene/ | Uniprot | Theoretical | Theoretical | No. of | Protein | Protein | PSORTb | PSORTb | COGb |
|---|---|---|---|---|---|---|---|---|---|---|---|
| locus tag | accession no. | protein MW | protein PI | peptides | scores | score | localization | probability | |||
| matched | C.I. %a | ||||||||||
| PK1–PK2–PK3 | Carbohydrate uptake ABC transporter-2 (CUT2) family, ATP-binding protein | MBOVPG45_0018 | tr|E4PYW6| | 59.27 | 7.79 | 21 | 324 | 100 | Cytoplasmic Membrane |
9.99 | R |
| PK4 | Endoglucanase | MBOVPG45_0256 tpx/ |
tr|A0A059Y901| | 39.39 | 5.42 | 18 | 621 | 100 | Cytoplasmic | 7.50 | EG |
| PK6–PK7–PK11–PK12 | Thiol peroxidase (Tpx) | MBOVPG45_0640 tuf/ |
tr|A0A059Y3P0| | 18.47 | 5.44 | 15 | 774 | 100 | Cytoplasmic | 7.50 | O |
| PK8 | Elongation factor Tu (Tuf) | MBOVPG45_0411 uvrA/ |
tr|A0A059Y8L3| | 43.64 | 5.89 | 26 | 860 | 100 | Cytoplasmic | 9.97 | J |
| PK9 | UvrABC system protein A (UvrA) | MBOVPG45_0470 pta-1/ | tr|E4Q030| | 106.14 | 6.94 | 20 | 53 | 99.23 | Cytoplasmic | 7.50 | L |
| PK10 | Phosphotransacetylase (Pta_1) |
MBOVPG45_0153 milA |
tr|A0A059XYN4| | 34.37 | 6.10 | 23 | 1,090 | 100 | Cytoplasmic | 7.50 | C |
| PK10 | Putative membrane protein (MilA) | MBOVPG45_0710 smc/ |
tr|E4Q0M5| | 302.89 | 8.71 | 36 | 48 | 97.55 | Unknown | – | E |
| PK10 | Chromosome partition protein Smc (Smc) |
MBOVPG45_0520 | tr|A0A059XZ49| | 111.20 | 5.74 | 13 | 47 | 96.92 | Cytoplasmic | 7.50 | D |
aC.I. %, confidence interval.
b COGs database functional categories: (C) Energy production and conversion, (D) cell cycle control, cell division, chromosome partitioning, (E) amino acid transport and metabolism, (G) carbohydrate transport and metabolism, (J) translation, ribosomal structure and biogenesis, (L) replication, recombination and repair, (O) post-translational modification, protein turnover, chaperones, (P) inorganic ion transport and metabolism, (R) general function prediction only.