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. 2018 Mar 5;9:278. doi: 10.3389/fpls.2018.00278

FIGURE 4.

FIGURE 4

Transgenic activity analyses of CsVMV8L13, MUASCsV8CPL6, MUAS35SCPL1, CaMV35SL9, and CaMV35S2L11. (A) Southern blot analyses performed to investigate uidA gene copy number in transgenic tobacco lines. (B) GUS expression analyses of the above promoter constructs in 21-day old transgenic seedlings. (C) Each bar represents the relative fold difference of uidA transcript levels of the respective constructs. The mean relative fold differences obtained from three independent experiments with respective SD for each promoter construct was presented. (D) Images of X-Gluc treated whole seedlings (T2 generation) expressing GUS under the control of CsVMV8L13#7, MUASCsV8CPL6#2, MUAS35SCPL1#1, CaMV35SL9#3 and CaMV35S2L11#6 promoter constructs. Asterisks indicate level of significance where “∗∗” is more significant than “”.