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. 2018 Mar 6;9(2):e02425-17. doi: 10.1128/mBio.02425-17

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FIG 1 — In vitro selection of highly DRV-resistant variants using various infectious HIV-1 clones. The impact of four amino acid substitutions, V32I, L33F, I54M, and I84V, on the development of DRV resistance was examined. (A and B) rHIV^WT, rHIV^V32I/I54M, rHIV^L33F/I84V, and rHIV^{V32I/L33F/I54M/I84V} (A) and rHIV^V32I, rHIV^L33F, rHIV^I54M, and rHIV^I84V (B) were propagated in the presence of increasing concentrations of DRV in MT-4 cells. At the conclusion of each passage, cell-free supernatant was harvested from the culture and subsequently added to a following culture replenished with the same number of uninfected target cells, and the virus was further propagated. This passage was repeated every 1 to 3 weeks for a total of 17 to 50 weeks.