FIG 3 .

Nonparallel Ab responses to LPS and porins develop after immunization with STmGMMA. WT mice were immunized i.p. with 1 μg of GMMA for the times indicated. (A) B-cell ELISPOT assay used to assess IgM⁺ (left) and IgG⁺ (right) ASC against LPS (closed circles) and porins (open circles) in spleens 4 and 7 days after immunization. Numbers of spot-forming units (SFU) per million splenocytes are depicted in the graphs alongside representative pictures of wells after development. Each circle represents one mouse, and pooled results of three experiments are shown, except for the day 4 results, which are from two experiments with at least three mice per group. (B) Relative Ab titers, as determined by ELISA, in serum samples against LPS (closed circles, solid line) or porins (open circles, dashed line) from mice immunized with STmGMMA for the times indicated. Each circle represents the mean value of four mice ± the standard deviation. The dotted vertical line represents the time point of reimmunization (Re-imm). The Mann-Whitney U test with Bonferroni correction for multiple comparisons was used to determine significant differences between groups linked by bars. NS, nonsignificant; *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001.